Fabrication of nano- or micro-powder for hydroxyapatite particles derived from bovine bone

Use your smartphone to scan this QR code and download this article ABSTRACT Background: Hydroxyapatite (HA) from bovine bone has been shown to have bone regenerative capability and therefore has potential benefits in bone grafting. However, the extraction procedures are often time-consuming and labor intensive. Therefore, this study aimed to devise a simple process to fabricate HA particles from bovine bone with the potential to be used in bone tissue engineering. Methods: In this study, the simple process combined physical and chemical methods to remove numerous organic components, followed by annealing at 600oC to fabricate small crystal HA particles. The organic presence in the HA product was detected by Fourier-transform infrared (FTIR) spectroscopy. The particle size, interconnected porous structure, and the calciumto-phosphorousmolar ratio (Ca/P) were examined by X-ray powder diffractionmethod (XRD), scanning electron microscope (SEM), and energy dispersive X-ray (EDX) analysis. Results: The combination method completely removed the organic composition. The results of SEM, XRD, and FTIR demonstrated the formation and preservation of small (nanometer sized) crystal HA particles with the porous structure. The Ca/P ratio was similar to that for natural bone. Conclusion: Pure natural HA crystals could be extracted from bovine bone. The combination of chemical and thermal decomposition methods was effective in producing HA particles. The method proposed herein has the advantage of being simple and relatively inexpensive, thereby rendering it beneficial for large-scale application.


Hydroxyapatite
(HA, chemical formula Ca 10 (PO 4 ) 6 (OH) 2 ) is not only a biocompatible, osteoconductive, non-toxic, non-inflammatory, and non-immunogenic agent but also a bioactive agent that has the ability to form a bond with the surrounding bone tissue following implantation. It has been shown to grow and develop similarly to natural bone; its biocompatibility and bioactivity allow the mediation of biological processes [1][2][3] . HA interacts robustly with tissues on the interface, resulting in stimulation of bone accumulation. After implantation, HA is gradually dissolved; ionic calcium (Ca 2+ ), phosphate (PO 4 3− ), and hydroxyl group (OH − ) in the environment are precipitated on the interface, resulting in a new mineral HA layer which attaches to tissues 4 . The mineral components in animal bone and human bone are similar. Remarkably, bovine bone contains a predominant mass of HA (65-70%), compared to other animals, and bovine bone generally has a large bone mass and is in abundant supply 5 . Natural HA from bovine bone can be extracted by hydrothermal reactions and calcination at different temperatures. Through this extraction method, organic components in bones can be thermally decomposed and removed. Bone graft from animals can help alleviate the limitations of autologous bone graft 6 , reduce disease contamination, and reduce patient pain. However, infectious particles (prions) cause Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. Therefore, the use of bovine material for medical products and devices poses the question as to what degree such a material could be considered free of prions and what the risks are of disease transmission to humans. Therefore, bovine bone processing should be quite well-controlled to avoid disease transmission 7 . In general, there are two processes at present used to treat the bovine cortical bone. One uses a lowtemperature chemical extraction process, while the other uses a high-temperature (>1000 • C) process to remove residual organic materials.  11 .
In the study herein, we demonstrate a fabricating process from bovine bone using the combination of chemical and low-thermal decomposition method. The removal of inorganic substances was detected by Fourier-transform infrared (FTIR) spectroscopy. The final multiphase product with the main component of the HA crystals were observed by X-ray powder diffraction method (XRD), scanning electron microscope (SEM), and energy dispersive X-ray (EDX) analysis. The results suggest that HA particles derived from bovine bone by the proposed method did not change the natural microstructure nor bone composition, and thus should be further studied for fabricating advanced composites.

Materials
A femur (plexiform bone) of an adult bovine (which was bought from a local slaughterhouse) was removed of visible substances and tissues on the bone surface. Then, it was smashed into 3 x 3 cm sized fragments. Distilled water (2x), chloroform, ethanol and sodium hypochlorite were provided by Chemsol (Vietnam). The two commercial biomaterials used in this study (Geistlich Bio-Oss ® ) were derived from inorganic medullar bovine bone and were used as comparative samples.

HA fabrication method
The fabrication procedure consisted of three stages: Stage 1: Cleaning and boiling. Bone fragments were immersed in distilled water for 24 h and water was removed every 12 h. After that, the macroscopic adhering impurities and substances (which included the ligaments and tissues that stick on the bone) were shaved and removed. Raw bone was stewed for 12 h and water was replaced every 3 h. After boiling, the bone samples were dried for 12 h at 60 o C and then subjected to the milling process until a fine powder was generated. Figure 1 shows the flow chart of the cleaning and boiling stage. Stage 2: Organic solvent and detergent treatment. Each 1 g of bone grain was treated with 20 mL of ethanol: chloroform mixture (v/v), with magnetic stirring, for 24 h. The bone was washed using fresh distilled water and dried for 12 h at 60 o C. Again, grains of bovine bone were treated with 25 mL sodium hypochlorite (NaClO 4%), with magnetic stirring, for 24 h. Bone grains were soaked in distilled water for 72 h and water was removed every 2 h during the first 12 h, and then removed every 12 h for the next 60 h. Ultimately, they were dried at 60 o C for 12 h. Stage 3: Thermal treatment. Bone grains were annealed in an electric furnace at 600 o C for 3 h and then separated through a strainer with a hole size of 250 µm. The final bovine bone-derived HA powder was stored in zipper bags at room temperature. Figure 2 shows the flow chart of the second and third stages.

Characterization methods
FTIR spectroscopy (Bruker Tensor 27) was used to determine the specific oscillation of PO 4 3− and CO 3 2− groups and residual organic components in HA particles. For this measurement, the transmission IR spectra were recorded using the KBr pellet over the range of 4004000 cm −1 , with 1 cm −1 resolution averaging over 100 scans. XRD method (Bruker XRD-D8 Advance, UK) was used to determine the existence of hydroxyapatite and the average size of crystal HAs. Phase analysis was carried out at room temperature using Cu Kα at a scan speed of 0.5 o /min and a step scan of 0.02 o . The half-width of diffraction peak was calculated by Origin software (OriginLab ® ) and the size of crystals was calculated by the Scherrer equation. The surface morphology and crystal size of the extracted bovine bone HA was observed under optical microscope at 20000 magnification (x20k) and SEM analysis (Hitachi S-4800, Japan) at 20000, 60000 and 120000 magnification (x20k, x60k, x120k). Chemical analysis was also examined by EDX spectroscopy to confirm the components and ratio of different elements in the product.

General observations
The color of the product underwent obvious change throughout each stage. Instead of the yellow color (and strong smell) observed at the end of the first stage (Figure 3), the bone was yellowish-white in color (and odorless) at the end of the second stage. The final product was a bright white grain in various sizes (Figure 4).

FTIR results
The FTIR spectrum values of HA for an annealing temperature of at 600 o C are shown in Figure 6 and The FTIR spectra in Figure 6 (right) shows the alteration of the specific organic peaks in bone particle during the three stages of the procedure. The C-H oscillation peaks associated with the CH 2 groups (2926 cm −1 and 2856 cm −1 ), which were observed on the spectrum of bovine bone in the first stage (I), were diminished in the second stage (II) and did not appear on the FTIR spectrum of the third stage (III).

XRD phase results
The results of the XRD analysis for the treated bone as well as the two comparative samples are shown in Figure 7. Most of the specific peaks for hydroxyapatite in the Bio-Oss/IngeniOs commercial products also corresponded to those of the fabricated HA particles 12 . It was also revealed that there were no significant differences between the commercial HA and extracted HA from bovine bone.  (Figure 7).
The particle size of HA crystals was not exactly homogenous, but the particle average size was between 15 nm and 30 nm ( Table 2).    Figure 9 shows the porous structure of the extracted micro-HA particles as observed under x20k magnification under optical microscopy. The particle size of HA in our treated bone was 30 to 80 µm, with pore size averaging 2-5 µm in size. The reference SEM images of Geistlich Bio-Oss ® (left) and IngeniOs TM HA (right) were introduced in Figure 10 under magnifi-cation x50k (https://dental.geistlich-na.com/en-us/pr ofessionals/bone-substitutes/bio-oss/user-benefits). The SEM micrograph of HA extracted powder obtained from bovine bone is shown in Figure 10 (a, b and c). In Figure 11, the very small hydroxyapatite particles had rod shape-growth on the bone particle (growth in disorientation) and they agglomerated together in some parts. Moreover, the disoriented growth of HA particles on bone particle surface   resulted in a more porous structure. The average size of surface HA particles ranged from 50 nm to 0.2 µm.

EDX results
The results of the EDX analysis of extracted bovine bone are not presented in this study. The chemical composition of the material is shown in

DISCUSSION
In the study of Eny Kusriniand and Muhammad Sontang, the clean bovine bone samples were defatted in boiling water followed by sun drying to remove the organic substances. Samples were sintered in the range of 500-1400 o C. The bone became black upon sintering at 500 o C, gray at 600-800 o C, and white from 900 to 1100 o C 13 . In our study, we noted that the color of the bovine bone powder changed visibly before the sintering treatment. Changes in bovine bone powder were related to decomposition of organic matter in the bone powder which indicates the complete removal of organic matrix, such as collagen, protein, polysaccharides and lipids 6,13,14 . Before sintering, the color of the bovine bone powder at room temperature was yellowish white and almost odorless; it changed to bright white upon sintering at 600 o C. Our results are consistent other previous studies. Moreover, our study results emphasized that: (i) the yellow white and odorless products were not yet thermally treated, and (ii) the bright white product was observed at low annealing temperature.
The However, absorption peaks of the hydroxyl group (OH-) which represent HA chemical fomulation could not be seen in the two comercial products, but a clear peak was seen in our final product (at 3571 cm −1 ). In addition, the Bio-Oss material sample showed a peak that is likely associated with a CO 3 2− vibration while this peak was completely absent for IngeniOs 12 . Meanwhile, our final product showed many characteristic peaks for CO 3 2− . Research by Agnieszka Sobczak-Kupiec explained that this difference was due to physicochemical properties of synthetic and natural hydroxyapatite which vary considerably. The differences arise from both a different molar ratio of Ca/P and the fact that natural HA contains CO 3 groups and a small amount of foreign elements 17 . Substitutes (e.g. CO 3 2− ) are most likely a remnant of the original material and may be decomposed at a higher temperature. In our study, the diminution and disappearance of C-H oscillation peaks (2926 cm −1 and 2856 cm −1 ) at the second stage (II) and third stage (III) shows agreement with complete removal of organic composition and that HA crystals appeared in the extracted bone. On the other hand, the partial decomposition of HA to form α-tricalcium phosphate (α-TCP), β -tricalcium phosphate (β -TCP) or tetra-calcium phosphate (TTCP) functional groups were not observed in our study. FT-IR results might confirm that the organic compounds present in the bovine bone were eliminated by the proposed treatment methods. Sintering bovine    x20k (very small hydroxyapatite particles are seen growing on the particle surface), b) x60k (HA particles are seen to be of regular size and high density but display disorientation), and c) x120k (rod-shaped crystal HA particles are seen growing in disorientation in the porous structure).
above 600 o C might help to obtain pure mineral phase HA. XRD analysis determines the phase and purity of HA crystals. Barakat et al. (2009) stated that not all the standard hydroxyapatite peaks have been formed or shown in the raw bovine bone due to the presence of fibrous collagen which disperses the X-ray radiation 18 . In our study results, all the peaks corresponding to the standard hydroxyapatite are obvious in the spectra of the extracted bones which illustrates that the treatment processes have produced pure hydroxyapatite. The degree of sharpness of peaks in the extracted bovine bone spectra indicates the crystal formation of the HA and their high purity. The homologous spectrum between treated bone and Bio-Oss ® /IngeniOs TM demonstrates that the proposed process has not affected the chemical structure of HA present in the bovine bone. Moreover, in our study, the SEM micrograph results show that the particles had irregular shape (mostly rod shape), showed disorientation, and agglomerated together in some regions. The SEM analysis revealed a porous architecture which maintained the spongy structure of natural bone. On the other hand, the size of HA particle (30-80 µm) was 10 times larger than the size of the pores (2-5 µm); these were in agreement with the particle size and pore ratio for Bio-Oss ® . The morphology of the HA extracted powder exhibited a porous network due to the removal of organic materials from the bone that are suitable for bone in-growth to form the osseous bed 19 .
The EDX analysis confirmed that the obtained product from the proposed extraction process was hydroxyapatite. Ca, P and O elements in the present study are indeed the major constituents of extracted bone. C.Y. Ooi et al. (2007) showed in their study that the inorganic phases of annealed bovine bone were composed mainly of Ca and P as the major constituents, with some minor components comprising of Na, Mg, O and C. The Ca/P ratio, in their study, of bovine bone annealed at 600 o C was approximately greater than 2.0 19 . According to the chemical formula of the standard hydroxyapatite, the calcium to phosphorous molar ratio is approximately 1.67 20 . The Ca/P of our product is the same as that for Bio-Oss material (Ca/P =1.58) while lower than that for IngeniOs material (Ca/P =1.62) 12 .
In the study herein, calcium, phosphorous and oxygen elements were found to be the predominant constituents of the treated bone. The Ca/P ratio for hydroxyapatite obtained by our study method is 1.56 which is similar to that for Bio-Oss material (1.58) and which lies within the acceptable range for hydroxyapatite 20 .

CONCLUSION
Calcination is one of important processes which affect the characteristics, efficiency, pure phase and size of the HA material. The combination of chemical solvent heating can lead to energy reduction or elimination, lowered cost and higher performance. Our study confirms that bovine bone HA can be extracted from the combination of methods which includes physical and chemical means, and sintering upon 600 o C. The organic components were completely removed out of the bone, resulting in interconnected porous structures of the bone particle. Our fabrication method is, therefore, practical for producing HA from bovine bone.

HA:
Hydroxyapatite FTIR: Fourier Transform Infrared XRD: the X-ray powder diffraction method SEM: Scanning Electron Microscope EDX: energy dispersive X-ray spectroscopy TCP: tricalcium phosphate