The phytochemical investigation from n-hexane extract of the lichen Roccellamontagnei

Use your smartphone to scan this QR code and download this article ABSTRACT Introduction: Roccella montagnei is widely distributed in subtropical regions. As the continuous study on the hexane extract of Roccella montagnei lichen, the isolation and structural determination of five compounds were addressed. Method: The crude extract was obtained from the dried lichen powder's extraction at room temperature. The n-hexane, n-hexane-ethyl acetate, and ethyl acetate extracts were obtained by the liquid-liquid partition method. The organic compounds were isolated from n-hexane extract by silica gel and Sephadex LH-20 column chromatography. Their chemical structures were identified by the NMR and HR-ESI-MS data analysis and the comparison of their NMR data with the published data. Results: Five compounds were isolated and chemically structural identified, consisting of 3β -hydroxy-7α -methoxystigmast-5-ene (1), sekikaic acid (2), lichenxanthone (3), (+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin (4), and ar-turmerone (5). Conclusion: To the best of our knowledge, except 3 which was reported from this species for the first time, four isolated compounds left did not known to be present in Roccella genus before.


INTRODUCTION
Rocclella genus distributes in the subtropical regions, Mediterranean and mainly in the Northern hemisphere 1 . Roccella montagnei species is commonly found along the Coromandel coast and in the mangrove forest in Indian and in the South of Vietnam 2 . The previously chemical investigations on this species showed the presence of depside, depsidone, quinone, sterol, usnic acid derivatives, and phenolic compounds [3][4][5] . The bioactive assessments on extracts and isolated compounds from this species revealed antiinflammatory, antimicrobial, anti-arthritic, antioxidant, and anticancer activities 2,4,6,7 . The phytochemical investigation on this species was further studied on n-hexane extract led to the isolation and structural elucidation of 5 compounds.

General experimental procedures
NMR spectra were recorded on Bruker Avance at 500 MHz for 1 H-NMR and 125 MHz for 13 C-NMR or on Bruker Avance III at 400 MHz for 1 H-NMR and 100 MHz for 13 C-NMR. In addition, the HR-ESI-MS spectra were acquired on a Bruker MicrOTOF-Q 10187. The NMR and MS spectra were done at the Center Analysis Laboratory of the University of Science, Vietnam National University (VNU)-Ho Chi Minh City and the Institute of Drug Quality Control Ho Chi Minh city.

Plant material
The lichen Roccella montagnei was collected at Tuy Phong District, Binh Thuan Province, Vietnam, in June 2018. The scientific name of the lichen was authenticated by Dr. Holger Thüs, Life Science Department, The Natural History Museum, Cromwell Road, SW7 5BD London, England, UK. In addition, a voucher specimen (No US-B024) was deposited in the Herbarium of Department of Organic Chemistry, Faculty of Chemistry, University of Science, VNU HCM.

RESULTS
From the n-hexane extract of the lichen Roccella montagnei, collected at Binh Thuan Province, five compounds, 1 (10 mg), 2 (9.5 mg), 3 (11 mg), 4 (7 mg), and 5 (8.7 mg), were isolated. Their physical properties and spectroscopic data were performed as follows.   Figure 2. The 13 C-NMR spectrum showed 22 signals consisting of twelve signals of two benzene rings from 99.7 to 165.8 ppm, two carboxyl carbons at δ C 169.3 (C-7) and 174.2 (C-7 ′ ), two methoxy carbons at δ C 55.9 (4-OCH 3 ) and 56.5 (4 ′ -OCH 3 ), and six final carbon signals from 14.6 to 39.2 ppm belonging to two n-propyl chains. The position of two methoxys were C-4 and C-4' , which were confirmed by HMBC correlations of the methoxy protons to carbons at δ C 165.4 (C-4) and 156.2 (C-4 ′ ). The HMBC correlations of H-8 to carbon C-1, C-5, and C-6, of H-8' to carbons C-1' , C-5' , and C-6' determined the positions of two n-propyl groups at C-6 and C-6' . All other HMBC correlations confirmed the chemical structure of 2. Additionally, the comparison of NMR data of 2 with the published data 9 showed good compatibility; therefore, 2 was assigned as sekikaic acid. Compound 3 was isolated as a white crystal. The 1 H-NMR spectrum showed a signal at δ H 13.39 (s, 1-OH) of the proton of a phenolic hydroxy group which was chelated to an ortho-carbonyl group, two metadoublet signals which had the multiplet skewing effect together at δ H 6.30 (d, 2.0 Hz, H-2), 6.34 (d, 2.4 Hz, H-4) of a tetra-substituted benzene ring, two other meta-doublet signals δ H 6.69 (d, 2.4 Hz, H-5), 6.67 (d, 2.0 Hz, H-7) of the second tetra-substituted benzene ring. The moderate magnetic zone revealed signals of two methoxy groups at δ H 3.87 (s, 3-OCH 3 ) and 3.90 (s, 6-OCH 3 ). The final proton signal at δ H 2.85 (s, H-9) was belonged to a methyl group connected to a benzene ring. Besides, the NMR data of 3 showed highly relevant to the published data 10 , 3 was thus determined as lichenxanthone. Compound 4 was isolated as a colorless needle crystal. The HR-ESI-MS of 4 showed the sodiated ion peak at m/z 245.0794 [M+Na] + (calcd. for C 12 H 14 O 4 Na, 245.0790) which deduced its molecular formula to be C 12 H 14 O 4 . The 1 H-NMR spectrum displayed a singlet proton signal at δ H 11.20 (s, 8-OH) of a phenolic hydroxy group which was chelated to an ortho-carbonyl group. Two broad-singlet proton signals at 6.31 (brs, H-5) and 6.21 (brs, H-7) suggested the presence of a 1,2,3,5-tetra-substituted benzene ring. A very broad-singlet signal integrated one proton at δ H 6.00 (brs, 6-OH) was deduced to a hydroxy group which did not exist the intramolecular hydrogen bond. There was a multiplet signal at δ  olefinic proton at δ H 6.02 (1H, s, H-11) of a methine group =CH-attached to two quaternary carbons. At the high magnetic field, the proton spectrum displayed signals at δ H 2.10 (d, 1.6 Hz, H-13) and 1.85 (d, 1.6 Hz, H-14), which possessed HMBC correlations to the same olefinic carbons at δ C 124.2 (C-11), 155.3 (C-12). These signals suggested the presence of -CH=C(CH 3 ) 2 moiety. A methyl proton signal resonated at 2.31 (s, H-1) was suggested to attach to the benzene ring (as para-CH 3 C 6 H 4 -), which was further confirmed by HMBC cross-peaks of this proton signal to aromatic carbons at δ C 135.9 (C-2) and 129.2 (C-3/C-7). A proton signal appearing as a doublet, integrated three-protons at 1.24 (d, 6.8 Hz, H-15) was assigned to the methyl group attached to the other methine group. This methyl proton signal showed HMBC correlations to a quaternary aromatic carbon C-5 at 143.5 (C-5), a methine carbon C-8 (35.4), and a methylene carbon C-9 (52.8), which demonstrated the presence of CH 3 -CH(C 6 H 4 CH 3 )-CH 2 -fragment. The 13 C-NMR spectrum showed a ketone carbon signal shielded at 200.1 (C-10), deduced to be the ketone group conjugated to the double bond. It corresponded to the sole quaternary olefinic carbon C-12 shifted to the higher frequency at 155.3 ppm. All the rest of HMBC correlations supported the chemical structure as shown. Additionally, the comparison of its NMR data to those reported in the literature 12 gave further evidence to confirm the chemical structure of 5 as ar-turmerone. The optical rotation value of [α] 20 D = 0 suggested 5 to be a racemic mixture of ar-turmerone. Ar-turmerone was known as the major bioactive compound of Curcuma longa species and possessing anti-inflammatory and neuroprotective property 13 .

CONCLUSION
From the n-hexane extract of the lichen Roccella montagnei, five compounds were isolated consisting of a sterol (3β -hydroxy-7α-methoxy-stigmast-5-ene), a depside (sekikaic acid), a xanthone (lichenxanthone), a coumarin derivative ((+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin), and an ar-turmerone (5). Their chemical structures were elucidated by NMR, MS spectroscopic data analysis, optical rotations, and the comparison to the published data. Four isolated compounds 1, 2, 4, and 5 were reported to presence in the Roccella genus for the first time while 3 had been already isolated from other species of this genus. The ongoing studies on this species are in progress.