TY - JOUR AU - Khanh Pham AU - Nguyen Nhut AU - Nguyen Cuong PY - 2020/09/04 Y2 - 2024/03/29 TI - New method for preparing purity β-D-glucans (beta-Glucan) from baker’s yeast (Saccharomyces cerevisiae) JF - Science and Technology Development Journal JA - STDJ VL - 23 IS - 3 SE - Section: NATURAL SCIENCES DO - https://doi.org/10.32508/stdj.v23i3.2051 UR - http://stdj.scienceandtechnology.com.vn/index.php/stdj/article/view/2051 AB - Introduction: β-D-glucans (beta-Glucan), a water-soluble polysaccharide with diversity physiological activities for applications in food and pharmaceutical industries.Methods: In this paper, we report the use of ionic liquid 1-butyl-3-methyl-imidazolium chloride [BMIM]Cl on the extraction and isolation of β-glucan from baker's yeast Saccharomyces cerevisiae. The β-D-glucans precipitated by adding water into the solution and obtained by filtration or centrifugation were pure, cleaned, and free of cell membranes.Results: The beta-glucan was obtained as white precipitates after adding water into the mixed solution. The 1D and 2D-NMR spectrum and titration methods applied for qualitative and quantitative determination showed that the beta-glucan product contained 78.2% 1,3-β-D glucan with 98.4% purity.Conclusion: This method can be used to prepare purified beta-glucan from baker’s yeast. ER -