TY - JOUR AU - Thao Nguyen AU - Dung Dang PY - 2023/04/15 Y2 - 2024/03/29 TI - Molecular cloning and isolation of a recombinant alpha-Momorcharin in E. coli against Pyricularia oryzae JF - Science and Technology Development Journal JA - STDJ VL - 26 IS - 1 SE - Section: NATURAL SCIENCES DO - https://doi.org/10.32508/stdj.v26i1.4023 UR - http://stdj.scienceandtechnology.com.vn/index.php/stdj/article/view/4023 AB - Introduction: Alpha-Momorcharin (α-MMC) is a member of the ribosome-inactivating protein (RIP) family that has been widely used as an antitumor, antiviral and antifungal agent.Methods: In this study, the codons of DNA encoding α-MMC were optimized for expression in E. coli and cloned into the pET-28a(+) vector. The protein was then expressed in E. coli strain BL21 (DE3) and purified by nickel affinity chromatography.Results: Under IPTG induction, α-MMC was expressed at approximately 50% of the total protein, showing high-level recombinant protein expression in E. coli. A high amount of purified α-MMC (70 mg) was isolated from 1 L LB culture medium of E. coli BL21 (DE3) with approximately 95% purity. Interestingly, α-MMC inhibited the mycelial growth of Pyricularia oryzae in a concentration-dependent manner.Conclusion: Using a microbial system for α-MMC expression provides a promising method for the design of a new agent against pathogens. ER -