TY - JOUR AU - Tuom Truong AU - Trang Phan AU - Linh Le AU - Dung Nguyen AU - Hoang Nguyen AU - Dung Dang PY - 2018/11/09 Y2 - 2024/03/29 TI - Engineering yellow fluorescent protein probe for visualization of parallel DNA G-quadruplex JF - Science and Technology Development Journal JA - STDJ VL - 21 IS - 3-4 SE - Natural Sciences - Research article DO - https://doi.org/10.32508/stdj.v21i3.461 UR - http://stdj.scienceandtechnology.com.vn/index.php/stdj/article/view/461 AB - Introduction: The formation of G-quadruplex plays a key role in many biological processes. Therefore, visualization of G-quadruplex is highly essential for design of G-quadruplex-targeted small molecules (drugs). Herein, we report on an engineered fluorescent protein probe which was able to distinguish G-quadruplex topologies.Methods: The fluorescent protein probe was generated by genetically incorporating yellow fluorescent protein (YFP) to RNA helicase associated with AU-rich element (RHAU) peptide motif. Results: This probe could selectively bind and visualize parallel G-quadruplex structure (T95-2T) at high affinity (Kd~130 nM). Visualization of the parallel G-quadruplex by RHAU-YFP could be easily observed in vitro by using normal Gel Doc or the naked eye.Conclusion: The YFP probe could be encoded in cells to provide a powerful tool for detection of parallel G-quadruplexes both in vitro and in vivo.  ER -