TY - JOUR AU - Phuong Huynh AU - Thanh Huynh AU - Nam Vo AU - Anh Nguyen AU - Hoang Nguyen AU - Trang Phan AU - Thong Nguyen AU - Giang Pham PY - 2015/12/30 Y2 - 2024/03/29 TI - Establishment of a Multiplex – PCR protocol for detection of Y chromosome microdeletion in Azoospermia male patients JF - Science and Technology Development Journal JA - STDJ VL - 18 IS - 4 SE - Natural Sciences - Research article DO - https://doi.org/10.32508/stdj.v18i4.904 UR - http://stdj.scienceandtechnology.com.vn/index.php/stdj/article/view/904 AB - Microdeletion on the Y chromosome is one of the causes that makes men infertile, accounting for 2-10 % of all infertility cases, and occurs frequently at 3 regions of the Ychromosome long arm namely AZFa, AZFb and AZFc (azoospermia factor). Currently, the diagnosis of microdeletion on the Y chromosome is almost mandatory in institutes and centers for infertility diseases before selecting treatment or assisting methods. To detect microdeletion in AZF, SRY and ZFY regions, the current approach is a Multiplex – PCR assay offering by European Academy of Andrology/European Molecular Genetics Quality Network (EAA/ EMQN). However, the drawback of this method is the PCR products posess similar size and then the DNA electrophoresis bands were very close on gels causing the difficult in diagnosis. Therefore, in this study, we have redesigned primer pairs matching with genes that were recommended by EAA/EMQN but the PCR products are clearly different in sizes, making the DNA electrophoresis bands take apart further to facilitate the diagnosis. Besides, we have also created recombinant plasmids carrying the marker genes for the control sample in kits. ER -