Science and Technology Development Journal 2018-11-15T20:50:38+00:00 Phuc Van Pham Open Journal Systems Analysis of 12C+12C scattering using different nuclear density distributions 2018-11-15T20:50:38+00:00 Nguyen Dien Quoc Bao Le Hoang Chien Chau Van Tao Trinh Hoa Lang <p>Elastic <sup>12</sup>C+<sup>12</sup>C angular distributions at three bombarding energies of 102.1, 112.0 and 126.1 MeV were analyzed in the framework of optical model (OM) and compared to the experimental data. The reality of the OM analysis using the double folding potential depends on the chosen nuclear density distributions. In this work, we use two available models of nuclear density distributions obtained from the electron scattering experiments and the density functional theory (DFT). The OM results show that the former gives better description of the <sup>12</sup>C nuclear density distribution than the latter. Therefore, the DFT should be worked on for improving the nuclear density description of <sup>12</sup>C in the future.</p> 2018-10-16T00:00:00+00:00 ##submission.copyrightStatement## Engineering yellow fluorescent protein probe for visualization of parallel DNA G-quadruplex 2018-11-15T20:50:37+00:00 Tuom TT Truong Trang PT Phan Linh TT Le Dung H Nguyen Hoang D Nguyen Dung Thanh Dang <p><strong>Introduction</strong>: The formation of G-quadruplex plays a key role in many biological processes. Therefore, visualization of G-quadruplex is highly essential for design of G-quadruplex-targeted small molecules (drugs). Herein, we report on an engineered fluorescent protein probe which was able to distinguish G-quadruplex topologies.</p> <p><strong>Methods</strong>: The fluorescent protein probe was generated by genetically incorporating yellow fluorescent protein (YFP) to RNA helicase associated with AU-rich element (RHAU) peptide motif.</p> <p><strong> Results</strong>: This probe could selectively bind and visualize parallel G-quadruplex structure (T95-2T) at high affinity (Kd~130 nM). Visualization of the parallel G-quadruplex by RHAU-YFP could be easily observed in vitro by using normal Gel Doc or the naked eye.</p> <p><strong>Conclusion</strong>: The YFP probe could be encoded in cells to provide a powerful tool for detection of parallel G-quadruplexes both <em>in vitro</em> and <em>in vivo</em>.</p> <p>&nbsp;</p> 2018-11-09T00:00:00+00:00 ##submission.copyrightStatement## Improving hairy root induction of Urena lobata L. by Agrobacterium rhizogenes ATCC 15834 by some factors 2018-11-15T20:50:35+00:00 Vu Thi Bach Phuong Pham Thi Anh Hong Quach Ngo Diem Phuong <p><strong>Introduction</strong>: Our previous study showed that <em>Urena lobata L.</em> hairy root is a potential pharmaceutical source for type 2 diabetes treatment. In order to improve the transformation efficacy and the quality of hairy roots, this study examined the effects of several factors including age, parts of plants, infection time and culture medium in inducing hairy roots in <em>Urena lobata L. </em></p> <p><strong>Methods</strong>: In this study, we investigated four factors to improve the hairy root induction in <em>Urena lobata L.</em> These factors include: age of plant (15-day-old in vitro plants, 45-day-old<em> in vitro</em> plants and after two subculture generations plants), different parts of plant (roots, stems, and leaves), infection time (10, 20 and 30 minutes), and culture medium (Murashige and Skoog (MS), Gamborg B5 medium (GB5) and Woody plant medium (WPM)). All experiments were repeated three times, with uninfected leaf explants of 15-day-old <em>in vitro</em> as the negative control. The transformation frequency and the fresh biomass of hairy roots were recorded at four weeks after infection.</p> <p><strong>Results</strong>: The results showed that the optimized procedure which used 15-day-old<em> in vitro</em> plants, the leafy part, the infection time of 10 minutes and culture in the WPM medium was better than the original procedure. The optimized procedure achieved a transformation frequency of 100%. In addition, the fresh biomass of hairy roots formed on an explant in the optimized procedure was 3.2 times higher than the ones induced by the original procedure.</p> <p><strong>Conclusion</strong>: The results showed that the optimized procedure was more effective than the original procedure in inducing <em>Urena lobata</em> hairy roots.</p> <p>&nbsp;</p> 2018-11-13T14:47:45+00:00 ##submission.copyrightStatement##