Abstract

Callus was initiated from leaves taken from 3 - 4 week - old plantlets of Solanum tuberosum AVRDC1297.19, on a MS basal medium supplemented with 2mg/l NAA and 0,5mg/1 BA. The aim of this work was to obtain somatic embryos of Solanum tuberosum AVRDC1297.19 through callus formation and identify the main histological and physiology characteristics associated with embryogenesis. Somatic embryos should begin to appear after 1-week passage on a MS medium supplemented with 2mg/l zeatin and then on a hormon free medium. The role of the plant regulators auxin and cytokinins (zeatin, kinetin and benzyladenin) on embryogenesis of Solanum tuberosum has been studied.