Abstract

Bifidobacterium bifidum or Lactobacillus casei were added independently into ice cream with or without homogenization. The viability of probiotic bacteria during storage, in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) was investigated. The results showed that the viability of Bifidobacterium bifidum and Lactobacillus casei in ice cream samples was significantly decreased during 100 days of storage. The homogenization in ice cream processing was a significant effect on the survival of L.casei and B.bifidum during frozen storage time. In addition, frozen storage time affected the viability of these strain when incubating in SGF and SIF conditions. The longer storage time, the more sensitive probiotic in SGF and SIF condition. The result also indicated, in SGF condition, the difference between homogenization and non-homogenization samples was only statistically significant (p<0,05) after 100 days of storage.