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Abstract
Biomass of Lactobacillus acidophilus was optimized in the tofu wastewater medium by Plackett-Burman matrix and Response surface methodology – Central composite design (RSM-CCD). The highest biomass was 0.73 g/100mL at 106 CFU/mL of initial density of L. acidophilus in tofu wastewater medium, with 0.37% (NH4)2SO4 and 17.59% sucrose. L. acidophilus produced maximum value of biomass after 20 hours of incubation at 37°C. There was a similar proportion at 96.05% in comparison to experimental value and optimization theory, approximatly 93.58% L. acidophilus biomass in MRS medium. As a result, optimization model could be applied in biomass production of L. acidophilus to enhance comercial value and contribute to environmental protection.
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Article Details
Issue: Vol 17 No 2 (2014)
Page No.: 60-72
Published: Jun 30, 2014
Section: Engineering and Technology - Research article
DOI: https://doi.org/10.32508/stdj.v17i2.1360
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Ly, H., & Nguyen, H. (2014). Plackett-Burman matrix and response surface methodology-central composite design for optimization of Lactobacillus Acidophilus biomass production. Science and Technology Development Journal, 17(2), 60-72. https://doi.org/https://doi.org/10.32508/stdj.v17i2.1360
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