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VNUHCM Journal of

Science and Technology Development

An official journal of Viet Nam National University Ho Chi Minh City, Viet Nam since 1997

ISSN: 1859-0128
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STUDY ON SHOOT REGENERATION FROM LEAF CALLUS OF TETRACERA SCANDENS L.

Ngoc Thi Bich Pham 1, *
Hoang Ngo Phan 1

  1. University of Science, VNU-HCM
Correspondence to: Ngoc Thi Bich Pham, University of Science, VNU-HCM. Email: pvphuc@hcmuns.edu.vn.
Volume & Issue: Vol. 12 No. 7 (2009) | Page No.: 79-85 | DOI: 10.32508/stdj.v12i7.2269
Published: 2009-04-15

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Copyright © 2009 by the author(s).

Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Tetracera scandens is an important herb, used to treat some diseases, such as gout, kidney diseases... The callus is formed from leaf on MS medium (Murashige & Skoog, 1962) supplemented with 2.5mg/l 2,4-D and 0.5mg/l BA. Before induced shoot regeneration, the callus had grown on MS medium supplemented with 0.5mg/BA and 0.5mg/l GA3. Shoot generation is actually induced on MS medium supplemented with 0.7mg/l BA and 0.1mg/l IAA. The number of shoot regeneration is about 26/callus (from 0,3cm' leaf tissue). Shoot origin, role of endogenous hormones and respiration rate in shoot regeneration were analysis.

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