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Abstract
The plasmid PSP-luc+, which contains a firefly luciferase gene, is Transformed into three E. coli host strains (JM109, DH5a and BL21(DE3)pLysS) to generate Juciferase - producing recombinant clones. Among the three obtained clones, the BL2IDE3)pLyss/pSP-luc+ clone showed the highest luciferase activity. The clone produced highest luciferase activity after 24 - 27 hours of shaking culture in LB medium. Effects of buffers and stabilizing agents such as glycerol and ammonium sulfate on luciferase activity were examined.
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Article Details
Issue: Vol 5 No 7&8 (2002)
Page No.: 44-50
Published: Aug 31, 2002
Section: Article
DOI: https://doi.org/10.32508/stdj.v5i7&8.3427
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Minh Tri, V., & Linh Thuoc, T. (2002). ESTABLISMENT OF LUCIFERASE - PRODUCING RECOMBINANT CLONE FOR THE IN VITRO BIOLUMINESCENCE REACTION. Science and Technology Development Journal, 5(7&8), 44-50. https://doi.org/https://doi.org/10.32508/stdj.v5i7&8.3427
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