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Enhanced survival of spray-dried microencapsulated Lactobacillus casei in the presence of mix-prebiotic

Dong My Lieu 1, *
Hoai Van Bui 2
Huong Thuy Nguyen 3
  1. Faculty of Food Technology, University of Food Industry, Ho Chi Minh city
  2. Center of experiment, University of Food Industry, Ho Chi Minh city
  3. Department of biotechnology, Ho Chi Minh city University of Technology, VNU-HCM
Correspondence to: Dong My Lieu, Faculty of Food Technology, University of Food Industry, Ho Chi Minh city. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 18 No. 2 (2015) | Page No.: 65-74 | DOI: 10.32508/stdj.v18i2.1060
Published: 2015-06-30

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Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

In this study, the effect of Galactooligosaccharide (GOS) (0% và 2% w/v) on microencapsulated L.casei in whey protein 10% (w/v) and maltodextrin 5% (w/v) by spray dry method were investigated. The physical characterization included analysis of morphology, particle size. The viable cell counts of the microcapsule were determined during storage for 50 days at 10oC and in simulated gastric fluid (SGF) and intestinal fluid (SIF). All microcapsules with (WMG sample) or without GOS (WM sample) in this study showed similar morphology and particle size, between 3 to 11µm. There no differences between WMG and WM sample in cell viability were observed. For spray dry conditions tested in this work the cell viable yield with WM sample about 86.14% whereas for WMG sample about 86.78%. The viability of the microcapsules in WMG and WM were reduced about 0.44 and 0.63 log(CFU/g), respectively and remained > 6 log(CFU/g) after 2 hour in SGF or 4 hour in SIF incubating. Microcapsules made by spray dry method with whey protein 10% (w/v) and maltodextrin 5% (w/v) as encapsulating which enhancing L.casei survival, maltodextrin’s role not only as a wall material in microencapsulation but also as a prebiotic potential, eventually leading to added GOS was not necessary.

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