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Human serum albumin immobilization on APTES coated magnetite nanoparticles

Thanh Trung Bui 1, *
Van Hung Pham 1
Hai Hoang Tran 2
  1. Medical and Pharmaceutical University,HCM
  2. Institute of Physics, HCM
Correspondence to: Thanh Trung Bui, Medical and Pharmaceutical University,HCM. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 17 No. 3 (2014) | Page No.: 41-51 | DOI: 10.32508/stdj.v17i3.1369
Published: 2014-09-30

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Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Magnetite nanoparticles were synthesized by co-precipitation of Fe2+ and Fe3+ with NH3.H20, and then 3- aminopropyltriethoxysilane (APTES) was coated on the magnetite nanoparticles by silanization reaction to achieve Fe3O4/APTES nanostructures. After modified by APTES, the nanostructures were activated by glutaraldehyde (GA) to obtain functional groups on the nanostructures surface. Human Serum Albumin (HSA) was immobilised on the Fe3O4/APTES and Fe3O4/APTES/GA nanostructures. The morphology and properties of the nanoparticles were characterized by transmission electron microscopy (TEM), Xray diffraction (XRD), vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), UV–vis spectroscopy and thermal gravimetric analysis (TGA). The HSA binding efficiency was estimated by the Bradford method.

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