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Abstract
In this study, we report results on the expression and purification of recombinant T4 DNA ligase. Plasmid pET16b-T4Dnl contains the gp30 gene which encodes for T4 DNA ligase. The target protein is fused with 10xHis tag to facilitate the purification and recovery. pET16b-T4Dnl was transformed into E. coli BL21(DE3) and then induced the expression of 10xHis-T4Dnl by IPTG. The recombinant protein was purified by Ni-NTA chromatography and confirmed by SDS-PAGE and Western blot. The activity of purified protein was tested by joining DNA λ/HindIII.
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Article Details
Issue: Vol 14 No 3 (2011)
Page No.: 73-79
Published: Sep 30, 2011
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v14i3.1991
Copyright Info
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Duong, D., Luong, D., Nguyen, H., Tran, H., Dang, T., & Tran, T. (2011). EXPRESSION AND PURIFICATION OF RECOMBINANT T4 DNA LIGASE IN E. COLI. Science and Technology Development Journal, 14(3), 73-79. https://doi.org/https://doi.org/10.32508/stdj.v14i3.1991
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