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Abstract
The objective of this study is to screen Bacillus strains for high lipase production; from 10 strains of Bacillus subtilis OII (BS7) has ability the best lipase production. The maximum lipase was carried out using liquid culture medium at pH 7,0, substrate concentrations 1,2% (w/w) after 3 days. The optimum pH and temperature on lipase activity were found to be pH 10,0 and 60 oC respectively. Ion Mn2+, Ca2+ are the ions remain activity; active lipase was inhibited by Cu2+ , Zn2 ; solution of SDS 0,2 % decreases 95% activity. Molecular weight of lipase production by BS7 is approximate 23,8kDa. The results are preconditions for the research on recombinant lipase by Bacillus subtilis OII.
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Article Details
Issue: Vol 14 No 3 (2011)
Page No.: 64-72
Published: Sep 30, 2011
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v14i3.2053
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Tran, K., Le, H., & Ngo, N. (2011). SELECTION, PRODUCTION AND CHARACTERIZATION OF BACILLUS LIPASE. Science and Technology Development Journal, 14(3), 64-72. https://doi.org/https://doi.org/10.32508/stdj.v14i3.2053
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