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VNUHCM Journal of

Science and Technology Development

An official journal of Viet Nam National University Ho Chi Minh City, Viet Nam since 1997

ISSN: 1859-0128
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SELECTION, PRODUCTION AND CHARACTERIZATION OF BACILLUS LIPASE

Khoa Dang Tran 1, *
Huy Quang Le 1
Nghiep Dai Ngo 1

  1. University of Science, VNU-HCM
Correspondence to: Khoa Dang Tran, University of Science, VNU-HCM. Email: pvphuc@hcmuns.edu.vn.
Volume & Issue: Vol. 14 No. 3 (2011) | Page No.: 64-72 | DOI: 10.32508/stdj.v14i3.2053
Published: 2011-09-30

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Copyright © 2011 by the author(s).

Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

The objective of this study is to screen Bacillus strains for high lipase production; from 10 strains of Bacillus subtilis OII (BS7) has ability the best lipase production. The maximum lipase was carried out using liquid culture medium at pH 7,0, substrate concentrations 1,2% (w/w) after 3 days. The optimum pH and temperature on lipase activity were found to be pH 10,0 and 60 oC respectively. Ion Mn2+, Ca2+ are the ions remain activity; active lipase was inhibited by Cu2+ , Zn2 ; solution of SDS 0,2 % decreases 95% activity. Molecular weight of lipase production by BS7 is approximate 23,8kDa. The results are preconditions for the research on recombinant lipase by Bacillus subtilis OII.

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