CALLUS AND CELL SUSPENSION CULTURE OF DROSERA BURMANNI VAHL FOR QUINONE PRODUCTION

Abstract

Drosera burmanni Vahl, one of three Drosera species in Vietnam, has been successfully cultured in vitro. Our previous researchs have shown that extracts of Drosera burmanni Vahl contain bioactive compounds such as naphthoquinone, anthraquinone. To obtain cell biomass as well as increase secondary metabolites, callus and cell suspension culture of Drosera burmanni Vahl become extremely urgent. Therefore, in this study, we focused on building Drosera burmanni Vahl callus and suspension culture process to obtain quinone. Our results show that the most optimized medium for callus culture is Gamborg’s B5, saccharose 20g/l, casein 100 mg/l, PVP 1g/l. To induce callus culture, the best hormone’s concentration is 2,4-D 0,2 mg/l, NAA 0,2 mg/l. Growing callus and increasing cell biomass in suspension culture are the same culture type. The peak of growing phase is on 12th. HPLC analysis showed present of plumbagin, one of quinone bioactive compounds determined in Drosera species, on cultured cell suspension.