Open Access 
Downloads
Download data is not yet available.
Abstract
Tranformation of fish by-products to food and products with bioactivity brings economic benefits and reduces waste ratio in fishsery industry. This study focussed on the extraction and purification of protease from intestine of Basa catfish (Pangasius bocourti). The optimal conditions for protease extraction from Basa catfish intestine were as follows: intestine and buffer ratio-1/1(w/w); pH 9,5; temperature 35°C; extraction time is 10 minutes. Isopropanol was a suitable precipitating agent for protease purification. The optimal ratio of enzyme extract and iosopropanol was 15/85 (v/v). The alkaline protease recovery yield was 90,42% and the purity degree was 1,65.
Author's Affiliation
Article Details
Issue: Vol 9 No 11 (2006)
Page No.: 59-68
Published: Nov 30, 2006
Section: Article
DOI: https://doi.org/10.32508/stdj.v9i11.3123
Copyright Info
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Quoc Hien, T., & Viet Man, L. (2006). EXTRACTION AND PURIFICATION OF PROTEASE FROM BASA CATFISH INTESTINE (Pangasius bocourti). Science and Technology Development Journal, 9(11), 59-68. https://doi.org/https://doi.org/10.32508/stdj.v9i11.3123
Download Citation
Cited by
Article Statistics
HTML = 1110 times
Download PDF = 441 times
Total = 441 times
Download PDF = 441 times
Total = 441 times
Most read articles by the same author(s)
- Le Van Viet Man, Tran Quoc Huy, STUDY ON PROLONGATION OF WATERMELON FRESH-CUT SHELFLIFE BY OZONE TREATMENT , Science and Technology Development Journal: Vol 11 No 9 (2008)
- Ton Nu Minh Nguyet, Le Ngoc Lieu, Nguyen Thi Hien Luong, Le Van Viet Man, EFFECT OF INITIAL SULFUR DIOXIDE CONTENT IN MUST ON THE KINETICS OF WINE PRIMARY FERMENTATION, USING YEAST IMMOBILIZED IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 9 (2008)
- Bui Thanh Huyen, Le Van Viet Man, ALCOHOLIC FERMENTATION WITH DIFFERENT INITIAL GLUCOSECONCENTRATION USING IMMOBILIZED YEAST IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 12 (2008)
- Ton Nu Minh Nguyet, Nguyen Thi Hien Luong, Le Ngoc Lieu, Le Van Viet Man, INFLUENCE OF INITIAL TANNIN CONCENTRATION IN MUST ON THE KINETICS OF WINE FERMENTATION, USING YEAST IMMOBILIZED IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 12 (2008)
- Tran Quoc Hien, Le Van Viet Man, EXTRACTION AND PURIFICATION OF PROTEASE FROM BASA CATFISH INTESTINE (Pangasius bocourti) , Science and Technology Development Journal: Vol 11 No 11 (2008)
- Lai Quoc Dat, Le Van Viet Man, Nguyen Nhu Y, STUDY ON PROBABILITY OF SYRUP PRE-CONCENTRATION BY NANOFILTRATION , Science and Technology Development Journal: Vol 10 No 4 (2007)
- Le Van Viet Man, Pham Quoc Chuong, IMPROVEMENT OF FERMENTATION PERFORMANCE IN HIGH GRAVITY BREWING , Science and Technology Development Journal: Vol 10 No 6 (2007)
- Vu Thi Kim Hanh, Le Van Viet Man, STUDY ON CONTINUOUS HYDROLYSIS OF SUCROSE BY INVERTASE IMMOBILIZED IN ALGINATE GEL , Science and Technology Development Journal: Vol 10 No 11 (2007)
- Lai Quoc Dat, Le Van Viet Man, Vo Thi Luyen, STUDY ON INFLUENCE OF SOME TECHNOLOGICAL FACTORS ON HIGH GRAVITY BREWING , Science and Technology Development Journal: Vol 9 No 4 (2006)
- Le Van Viet Man, Tran Tham Minh Hoang, Nguyen Ngoc Tuyet Suong, STUDY OF BREWER YEAST AUTOLYSIS FOR INVERTASE EXTRACTION , Science and Technology Development Journal: Vol 9 No 12 (2006)
