Article
DETECTION OF POINT MUTATIONS ON PBP2X GENE OF SOME PENICILLIN RESISTANT STREPTOCOCCUS PNEUMONIAE STRAINS BY PCR-SSCP
Published:
2004-02-29
Abstract
We used PCR and SSCP (Single Strand Conformation Polymorphism) methods to detect point mutations in a 830 bp-fragment of pbp2x gene in 20 Streptococcus pneumoniae penicillin-resistant strains. Results showed that all the strains investigated bore more than two nucleotide modifications compared with the susceptible one.