Abstract

In the previous papers, the micrograting in cassava (using nodal segments of Manihot esculenta as stocks and M. glaziovii as scions), and the protoplast fusion between the two species (using PEG) were described. In this paper, attempts were made to explain the genetic variation between M. glaziovii and four cultivars of cassava, by using PCR-RAPD with thirty synthetic oligonucleotide primers. DNA was extracted from calli. These calli were initiated from leaves taken from 3-4 week-old in vitro plantlets of Manihot glaziovii and the four cultivars of Manihot esculenta, on a MS basal medium supplemented with 2mg/l 2,4-D and 0,5mg/l BA.