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Abstract

In the previous papers, the micrograting in cassava (using nodal segments of Manihot esculenta as stocks and M. glaziovii as scions), and the protoplast fusion between the two species (using PEG) were described. In this paper, attempts were made to explain the genetic variation between M. glaziovii and four cultivars of cassava, by using PCR-RAPD with thirty synthetic oligonucleotide primers. DNA was extracted from calli. These calli were initiated from leaves taken from 3-4 week-old in vitro plantlets of Manihot glaziovii and the four cultivars of Manihot esculenta, on a MS basal medium supplemented with 2mg/l 2,4-D and 0,5mg/l BA.



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Issue: Vol 7 No 7 (2004)
Page No.: 31-36
Published: Jul 31, 2004
Section: Article
DOI: https://doi.org/10.32508/stdj.v7i7.3228

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Ngo Hoang, P., & Trang Viet, B. (2004). A PRELIMINARY STUDY FOR THE ANALYSIS OF GENETIC VARIATION BETWEEN MANIHOT GLAZIOVII MUEL-ARG AND SOME CULTIVARS OF CASSAVA (M. ESCULENTA CRANTZ) USING PCR-RAPD. Science and Technology Development Journal, 7(7), 31-36. https://doi.org/https://doi.org/10.32508/stdj.v7i7.3228

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