Abstract

The process of mature coconut water fermentation by the complex of two bacteria strains Gluconacetobacter nataicola and Lactobacillus acidophilus in order to get high glucuronic acid concentration was studied. A Blackett – Burman matrix was designed to study the factors that affect glucuronic acid formation. After screening, four factors included G. Nataicola density, L. acidophilus density, sucrose concentration and temperature that have strongly contributed to glucuronic acid formation was selected to design a matrix for modeling optimal point. The result of Response surface methodology (RSM) with Central composite design (CCD) showed the maximum value of glucuronic acid concentration 94.23mg/l at 4.79 logCFU/ml G. Nataicola density, 5.34 logCFU/ml L. acidophilus density, 8.99 % sucrose and incubation at 31.96oC, respectively. The highest value of glucuronic acid concentration in the practical model was 94.23 mg/l.