Study on optimization of conditions for Cordyceps pseudomilitaris DL0015 culture

Abstract

Cordyceps biomas contains large number of biologically active substances in such as adenosine, cordycepin, polysaccharides, proteins… So many Cordyceps species have been cultured for biomedical or functional food. Studies on isolation, classification and optimum media for cultivating of Cordyceps pseudomilitaris DL0015 have been informed. In this followed study, optimization of culture conditions of this fungus for the proruction of high adenosine, polysaccharide, and protein biomass were checked using BCPharSoft. 19 treatments the BCPharSoft predicted had been tested. The results showed that the optimum input parameters were initial pH 6.7; light cycles day and night; temperature varies from at 25 ± 2oC/16 days followed by 8 ± 2oC/7 days and ended by 25 ± 2oC/7 days; output parameters for mycelial biomass, adenosine, polysaccharide, and protein were 19.98 g/l, 835.50 mg/Kg, 11.24%, and 0.27%, respectively.