Solid-state production of laccase by Pleurotus sp. applied in glyphosate degradation

Abstract

N- phosphonomethyl glycine (glyphosate) is the derivative of the amino acid glycine. Glyphosate is a broad-spectrum herbicide. Glyphosate is translocated via phloem system and moves from the roots to the growing parts of the plants. Many researches proved that lignin-modifying enzymes LME including laccases have ability to degrade glyphosate. Pleurotus sp. was cultured during 12 days in the media with coir dust, sesame residue, urea, saccharose, K2HPO4 and minerals. After 12 days, the media contain laccase with the activity of 0.227U/g. Moreover, Pleurotus sp. also grew in the media which contain glyphosate and made a decrease in this content from 50mg/kg to 10.5mg/kg after 12 days.