Open Access 
Downloads
Abstract
Labelling and traceability of genetically modified organisms (GMOs) are necessary for trade and regulation in the world and Vietnam. Cowpea Trypsin Inhibitor (CpTI) gene encodes a trypsin inhibitor which is considered as a suitable candidate for developing insect-resistant transgenic plants, especially transgenic rice lines originating from China. In this study, we established a real-time PCR protocol to detect the CpTI gene in transgenic rice. The protocol with CpTI-F and CpTI-R primers, 300 nM primers, 0.5 X SYBR Green I, annealing temperature at 62 0C showed the best results. Amplification efficiency is 94.64 % and the limit of detection is 50 copies. Moreover, PCR product of CpTI gene was cloned into pBluescript plasmid using as a positive control
Issue: Vol 18 No 2 (2015)
Page No.: 74-86
Published: Jun 30, 2015
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v18i2.1145
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Download PDF = 1451 times
Total = 1451 times
Most read articles by the same author(s)
- Nhut Nhu Nguyen, Le Van Bui, Hairy root induction in Catharanthus roseus by various strains of Agrobacterium rhizogenes isolated in Vietnam , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Phuong Hoang Phi Cung, Hao Thanh Nguyen, Le Van Bui, Study on the specificity of some molecular markers related to the cytoplasmic male sterility trait of vietnamese Chi Thien chilli pepper cultivar (Capsicum frutescens) , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Uyen Hong Thu Vu, Trong Huu Nguyen, Phuc Hoang Tran, Le Van Bui, Micropropagation of venus flytrap Dionaea muscipula , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Ha Van Tran, Phuong Hoang Phi Cung, Le Van Bui, Transformation of callus sugarcane (Saccharum officinarum L.) using Agrobacterium tumefaciens – Mediated method and regeneration of transgenic shoot , Science and Technology Development Journal: Vol 16 No 1 (2013)
- Phuong Ngo Diem Quach, Minh Thi Thanh Hoang, Yen Phi Le, Phung Kim Phi Nguyen, Le Van Bui, SCREENING AND ISOLATING AN ANTIOXIDANT FROM IN VITRO DROSERA INDICA L. EXTRACT , Science and Technology Development Journal: Vol 14 No 3 (2011)
- Nam Phuong Kieu, Hieu Le Trung Ho, Tuan Minh Tran, Thien Thi Di Do, Le Van Bui, INCREASE THE RATIO OF SEED GERMINATION BY USING METHYLOBACTERIUM SPP. , Science and Technology Development Journal: Vol 13 No 3 (2010)
