Introduction: The aim of this study is to determine appropriate parameters in the synthesis of syringic acid onto chitooligosaccharides (COSs) with an ascorbic acid/hydrogen peroxide redox pair in order to obtain the derivative with the highest grafting degree.
Methods: In this study, syringic acid grafted COSs, catalysed by an ascorbic acid/hydrogen peroxide redox pair were investigated. The synthesis conditions were investigated, including the mass ratio between syringic acid and COSs, pH, temperature and synthesis time. Characteristics of the derivative were evaluated by Thin Layer Chromatography (TLC), Ultraviolet-Visible (UV-vis) and Fourier Transform Infrared (FT-IR) spectroscopy. The activities of COSs and derivative were evaluated by antimicrobial ability.
Results: The results showed, that the best conditions for the synthesis were the mass ratio between syringic acid and COSs at 0.5:1, pH 5, temperature 27oC, for 6 hours with grafting degree at 32%. The TLC assay showed, that free ascorbic acid and syringic acid are not present in the product. The UV-vis and FT-IR data confirmed, that syringic acid was successfully conjugated onto COSs. Furthermore, the antibacterial assay showed that syringic acid grafted onto COSs had minimum inhibitory concentration against foodborne pathogenic bacteria at 1%.
Conclusion: The syringic acid onto chitooligosaccharides were successfully synthesized by free radical mediated grafting method with an ascorbic acid/hydrogen peroxide redox pair. The grafting degree of syringic acid onto COSs was greatly affected by many factors, including COSs, syringic acid, pH, as well as temperature and time of reaction. Moreover, the new derivative showed enhanced antibacterial capabilities, as compare to free COSs.
University of Science, Biochemistry, Faculty of Biology-Biotechnology, Vietnam National University Ho Chi Minh City (VNU-HCM); Center of Experiment and Practice, Ho Chi Minh City University of Food Industry (HUFI)
University of Science, Biochemistry, Faculty of Biology-Biotechnology, Vietnam National University Ho Chi Minh City (VNU-HCM); Lab of Enzyme Technology, University of Science, Vietnam National University Ho Chi Minh City (VNU-HCM)
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Bui, H., & Ngo, D.-N. (2019). The research determines appropriate parameters in the synthesis process of syringic acid grafted chitooligosaccharides. Science and Technology Development Journal, 22(3), 317-323. https://doi.org/https://doi.org/10.32508/stdj.v22i3.1289
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Science and Technology Development Journal (STDJ) (1859-0128) is the official journal of Viet Nam National University Ho Chi Minh City, Viet Nam, published by Viet Nam National University Ho Chi Minh City, Viet Nam. Science & Technology Development Journal is a multiple discipline science journal covering from natural science, engineering & technology, humanities, art, laws, economics, earth science, environment, social sciences and health sciences.