Abstract

The objective of this thesis is studying the condition of fermentation using Acetobacter xylinum on molasses medium and sugarcane juice medium. Multi-objective optimization method was used to optimized the factors of fermentation process in order to achieve the highest yield of Bacterial Cellulose. The results showed that pepton: 1.0%; glucose 2.78% ; pH 4.9, tempurature 30^oC in 107 hours were optimal fermentation conditions for molasses medium while pepton: 0.93% ; glucose: 3.65% ; pH 5.2; tempurature 30.5 ^oC in 102 hours were optimal fermentation conditions for sugarcane juice medium. These two media above can be used as new media for the culture of Acetobacter xylinum in comparision to coconut milk medium.