HEPATOPROTECTIVE EFFECT OF ISOLATED CONSTITUENT FROM POLYGONUM TOMENTOSUM AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY

Abstract

The ethyl acetate fraction (Et-F) of Polygonum tomentosum Willd was separated by silica gel vacuum chromatography to give 11 subfractions (PTE1-PTE11). The antioxidative activity of subfractions was determined by qualitative assay by TLC assays. The result of qualitative assay showed that all 11 subfractions had antioxidative activity. The results of ex vivo hepatoprotection of 11 subfractions showed that PTE7, PTE8 and PTE9 had higher ex vivo hepatoprotective activities than those of the other subfractions. PTE8 was further studied by silica gel chromatography to obtain A1 compound, which was identified as quercitrin. (To the best of our knowledge) This is the first report on the occurrence of quercitrin in P. tomentosum. Quercitrin had signifying both ex vivo and in vivo hepatoprotective effects on injury liver mice induced by carbon tetracloride (CCl4). The results in the present study indicated that P. tomentosum is a potential source of natural hepatoprotection.