STUDYING ON CALLUS FORMATION FROM CHILLI PLANTLET CAPSICUM SP. AND CAPSAICINOID ACCUMULATION IN VITRO

Abstract

Callus was initiated from hypocotyls and cotyledons explants of chilli Capsicum sp. in vitro on MS medium with 0,5 mg/l kinetin and 2,4-D /NAA (1,0; 1,5; 2,0; 2,5 and 3,0 mg/l). Callus from cotyledon explants was induced in the dark better than in the light, whereas callus from hypocotyl explants was initiated in the light better than in the dark. Callus was more friable and grew faster on medium with 2,4-D and kinetin. MS medium with 3,0 mg/l 2,4-D and 0,5 mg/l kinetin was optimal for the growth of callus from cotyledon explants. Besides, callus from hypocotyl explants grew best on MS medium with 1,5 mg/l 2,4-D and 0,5 mg/l kinetin. Capsaicinoid from callus which was determined by thin layer chromatography was recognized in all treatment experiments.