Abstract

Morinda citrifolia L. is a valuable medicinal plant, used to treat many diseases, such as sleeplessness, backache, high blood pressure... To study the shoot morphogenesis in Morinda citrifolia L. for propagation in the future, we examined the effects of BA, Zeatin and NAA on adventitious shoot formation of hypocotyl. The results showed that the regeneration of adventitious shoot comprised three steps of morphogenesis. In this process, Zeatin 1mg/l stimulated the formation of shoot primordia in dark (after a week), earlier than BA at the same concentration. Shoot generation was slow (after five weeks) on MS medium supplemented with 0,1mg/l and Zeatin 1mg/l. Roles of respiration rate and endogenous hormones were discussed to understand the physiological changes in the adventitious root formation.