NEW MODIFIED COTTON FIBER APPLY TO SEPARATE ECG AND EGCG FROM TEA EXTRACT

Abstract

Developed the new technique to modify cotton fiber by esterification with citric acid, the reaction was carried out in the tight flask and applied argon as anti-burning reagent. The optimum condition was 170oC, 5h and 3g of citric acid per 2g of cotton fiber, which result was up to 0.88 mol citric acid grafted on 1 mol glucose. The modified cotton fiber thereafter was applied to purify epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) from green tea polyphenol by column chromatography with the suitable mobile phase.