USING 17α - HYDROXY - 20β- DIHYDROPROGESTERONE (17,20P), PROGESTERONE (P) AND DESOXYCORTICOSTERONE ACETATE (DOCA) TO INDUCE OOCYTE MATURATION AND OVULATION IN VIVO OF GRASS CARP (Ctenopharyngodon idellus)

Abstract

After a priming with the combination consisted of 13.3 ug of [D-Ala6, Pro9 Net] - mGnRH (LRH-A, China) and 2 mg of Domperidone (Dom; Motilium-M, Jannsen, Thailand) per kg female, the resolving doses of 17α,20β-dihydroprogesterone (3; 4; 5mg/kg), Progesterone (10; 15; 20mg/kg) and Desoxycorticosteron acetat (7,5; 10; 15 mg/kg) for oocyte maturation and ovulation in vivo on a Grass carp (Ctenopharyngodon idellus) were tested. Results of the study indicated that optimal doses of 17,20; P and DOCA were 4; 20 and 10 mg/kg respectively. Result of oocyte maturation and ovulation in vivo of the fish by the optimal doses of the abovementioned steroid hormones mixed with LRH-A+Dom or Dom in a single injection brought low spontaneous spawn rate, however their fertilization and hatching rate were normal and quite satisfy the requirements of fish artificial propagation techniques.