STUDY ON SOMATIC EMBRYOGENESIS OF CALLUS IN POLYGONUM MULTIFLORUM THUNB. LEAF IN VITRO

Abstract

Somatic embryogenesis is a technology to propagation plant. Somatic embyos can be produced indirectly via callus. In this study, we observe somatic embryogenesis of callus in Polygonum multiflorum thunb. in vitro. Callus in the Murashide and Skoog medium (MS) supplied NAA 2mg/l and BA 0.5 mg/l after 8 weeks is used as the material for the research. It is transferred MS with 2,4 D 1 mg/l in 1 week and MS without plant growth regulator. After 2 weeks, globulars appear. To transfer globular to MS with NAA 0.5 mg/l, BA 0.5 mg/l and 10% coconut milk, heart and cotyledonary appear.