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Abstract
Pectinase from Aspergillus niger by solid state medium containing powered carot as an inducer obtained the highest activity after 48 hours. Separating enzyme on a gel filtration column packed with Bio gel P 30, which showed that one peak contained activity of pectinase, and with column flow rate of 15 ml/h the separation was better than 30 ml/h. Using cross flow membrane with speed-pump of 150 rpm at the input, the purification of enzyme (3.1 times before filtration) was higher than at rate of 200rmp (2,2 times before filtration).
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Article Details
Issue: Vol 11 No 8 (2008)
Page No.: 46-50
Published: Aug 31, 2008
Section: Engineering and Technology - Research article
DOI: https://doi.org/10.32508/stdj.v11i8.2673
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Ngoc Oanh, H., & Ngoc Hung, T. (2008). PURIFICATION OF PECTINASE FROM ASP.NIGER BY THE TECHNIQUES OF GEL FILTRATION AND CROSS-FLOW MEMBRANE. Science and Technology Development Journal, 11(8), 46-50. https://doi.org/https://doi.org/10.32508/stdj.v11i8.2673
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