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Abstract

In previous reports in this series, we found out the effective purificution process of urease from Viet Nam yellow soybean. The pure soybean urease powder (8.44UI/mg powder) from the purification process was used to determine some properties. Polyacrylamide gel 7,5% electrophoresis, non SDS and B-mercaptoetanol, compare with standard proteins from 35 000 – 400 000Da, showed Viet Nam soybean urease's molecular mass about 440 000 Da. The soybean urease was heated with SDS and B-mercaptoethanol, then SDS - acrylamide gel 10% electrophoresis, compare with standard proteins 10 000 - 250 000 Da, indicated that there exists a single Viet Nam soybean urease subunit species with a size of about 17 000 Da. Kinetic parameters of Viet Nam yellow soybean urease, determined according to Lineweaver – Burk equation, are Km = 5.3 mM and Vmax = 6.77*10 mm/min.



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Article Details

Issue: Vol 10 No 5 (2007)
Page No.: 41-50
Published: May 31, 2007
Section: Article
DOI: https://doi.org/10.32508/stdj.v10i5.2782

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Phu, L., & Cam Vi, N. (2007). DETERMINING MOLECULAR MASS AND KINETIC PARAMETERS OF VIETNAM YELLOW SOYBEAN UREASE. Science and Technology Development Journal, 10(5), 41-50. https://doi.org/https://doi.org/10.32508/stdj.v10i5.2782

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