QUANTITATIVE ANALYSIS OF THE URIC ACID COMPONENT IN HUMAN URINARY STONES USING FT-RAMAN SPECTROSCOPY

Abstract

Uric acid in human urinary stones has been determined quantitavely by the FT-Raman spectroscopy. Raman spectroscopy is a single-beam technique. This has placed limitations on its use for measurement of species concentrations. To overcome these problems, potassium sulphate (K2SO4) or potassium nitrate (KNO3) were added to the sample an appropriate quantity as an internal standard material. The sampling in the method requiring only a brief period of mixing and grinding (without compressing pellet) is shown to be particularly convenient. The results were compared to that obtained using IR absorption spectroscopy method.