SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION FROM CELL SUSPENSION CULTURE IN RICE (Oryza sativa L. cv. Bang Ngoc)

Abstract

Cell suspension culture is a useful source of somatic cell for plant embryogenesis and genetic manipulation studies. The development of a method for establishing and maintaining Oryza sativa L. cv. Bang Ngoc cell cultures with high generative capacity was the objective of this study. Callus was obtained from mature seeds, on medium with 2mg/l 2,4-D, 1mg/l NAA, and 0.5mg/1 BA. The callus was placed in 100ml Erlenmeyer flasks (0,5g per flask) containing 10 ml of MS medium adjusted to pH 5.8 and supplemented with 5mg/1 2,4-D, and 2% sucrose. The cultures were exposed to a 12-h photoperiod (2500 +500lux) at 282°C with shaking at 80rpm on a gyratory shaker. To test the generative capacity, callus was harvested from the cell suspension cultures and placed on N6 agar medium containing 2mg/1 BA and 0.5mg/1 NAA. Histological studies confirmed different stages of somatic embryogenesis. The planlets obtained were transferred to a simple and hormone-free MS medium to facilitate regeneration of normal roots.