Abstract

The plasmid PSP-luc+, which contains a firefly luciferase gene, is Transformed into three E. coli host strains (JM109, DH5a and BL21(DE3)pLysS) to generate Juciferase - producing recombinant clones. Among the three obtained clones, the BL2IDE3)pLyss/pSP-luc+ clone showed the highest luciferase activity. The clone produced highest luciferase activity after 24 - 27 hours of shaking culture in LB medium. Effects of buffers and stabilizing agents such as glycerol and ammonium sulfate on luciferase activity were examined.