CALLUS INITIATION FROM SHOOTS AND LEAVES OF POTATO (SOLANUM TUBEROSUM L.)

Abstract

Potato callus was initiated from buds and sprouts of tuber and from leaves of Solanum tuberosum L. The buds and sprouts of tuber were placed on MS medium supplemented with 0,4 mg/l 2,4-D and 0,8 mg/l BA, in the dark, at 22 'C. The leaves taken from 3-4 week-old plantlets were placed on MS medium supplemented with 2mg/l NAA and 0,5 mg/l BA, in the dark, at 22 °C. The purpose of this paper is to show the presence of some plant growth regulators (auxins, gibberellins and cytokinins) in the explants and their roles on callus formation from the specialized tissues. The results are believed to reflect the fact that the presence of auxins combined with cytokinins in medium initiates and maintains callus in undifferentiated and slow growing state.