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DETERMINATION THE CONDITIONS IN CULTURE AND TUBERIZATION IN VITROOF TORPEDO GRASS (PANICUM REPENS L.)

Nguyen Du Sanh 1
Volume & Issue: Vol. 2 No. 1 (1999) | Page No.: 27-33 | DOI: 10.32508/stdj.v2i1.3610
Published: 1999-01-31

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Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Torpedo grass (Punicum repens L.) is a weed species usually found on different environmental conditions, easily infected in vitro culture. Sterilization of samples could be made with calcium hypochlorite 10% solution for 15 to 25 minutes or HgCl2 0,1% for 10 to 20 minutes. Adding Tween 20 (0,15% v/v) in the calcium hypochlorite solution or HgCl, solution increases the efficacy of sterilization. A solution of HgCl2 0,1% plus Tween 20 (0,15% v/v) during 15 minutes sterilization goes a high result. The Murashige-Skoog's salts culture medium complemented Morel and Wetmore medium's vitamins, 4% sucrose and 2 mg/1 IBA is used. Root formation is good with this IBA concentration. In our first experiment of culture in vitro, using the culture medium supplemented with 25 mg/l AlCl3 plantlets grow well. The samples should be taken from the stem instead of the tuber or the rhizome. Torpedo grass tuberization succeeds only under complete darkness at 27°C in the culture medium containing plant growth substances. The tuberization is not induced in the light at 27°C or in dark at 22°C although the culture medium have been supplemented with plant growth substances. The full strength Murashige-Skoog's salts are not effective for Torpedo grass tuberization. The tuberization culture medium is a haft strength Murashige-Skoog's salts complemented Morel and Wetmore medium's vitamins, 8% sucrose and plant growth substances. Gibberellin completely inhibited whereas cytokinin and ABA induced tuberization. The tuberization rate is high (76%) with BA 20 mg/l. When NAA, BA and ABA are mixed at concentration 0,4:20:0,3 mg/1, the rate of tuberization is the highest (91%). The role of plant growth substances on the tuberization is also discussed in this paper.

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