A NOVEL EXTRACTION METHOD OF VINDOLINE AND CATHARANTHINE FROM THE CATHARANTHUS ROSEUS G.DON (APOCYNACEAE)

Abstract

Vindoline and catharanthine considered as mong the most active clinical drug in the treatment of high blood pressure and high concentration of glucose in blood, were extracted from the Catharanthus roseus G. Don (Apocynaceae). Moreover those two alkaloids are the components of the binary anticancer alkaloids vinblastine and vincristine. Several research teams investigated the methods of extraction of those alkaloids, but those process of extraction, about thirty, showed low efficacity and are no longer general and efficaceous method. In the present paper, we describe the isolation process of vindoline and catharanthine by successive extraction with Mc Ilvaine'buffer solution and then by chromatography on alumina with eluent toluene-chloroform (3:2). The alkaloids extracted were characterized by the spectrocopic method IR, UV, NMR and showed to be identical with the authentic sample.