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Abstract

FliC protein from Salmonella enteritidis is currently interested due to its immunologic adjuvant property for the novel generation of recombinant vaccines. To produce a source for further researches on the immune effects of FliC, we generated an Escherichia coli based on recombinant vector called pET-fliC which is ligated from fliC gene with NdeI and XhoI double digested pET vectors. The results of expression of recombinant FliC, which was induced by IPTG, were confirmed by SDS-PAGE and Western blot probed with anti-6xHis tag. With the purity above 95 %, this recombinant FliC can be used as a material source for next studies on evaluating the adjuvant property of FliC.



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Issue: Vol 19 No 4 (2016)
Page No.: 62-69
Published: Dec 31, 2016
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v19i4.652

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Tran, C., Nguyen, A., & Tran, H. (2016). Cloning, expression and purification of the recombinant FliC from Salmonella enteritidis. Science and Technology Development Journal, 19(4), 62-69. https://doi.org/https://doi.org/10.32508/stdj.v19i4.652

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