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VNUHCM Journal of

Science and Technology Development

An official journal of Viet Nam National University Ho Chi Minh City, Viet Nam since 1997

ISSN: 1859-0128
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Natural Sciences - Research article Open Access Logo

Cloning, expression and purification of the recombinant FliC from Salmonella enteritidis

Chau Thi Bao Tran 1, *
Anh Viet Nguyen 1
Hieu Van Tran 1

  1. VNUHCM-University of Science
Correspondence to: Chau Thi Bao Tran, VNUHCM-University of Science. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 19 No. 4 (2016) | Page No.: 62-69 | DOI: 10.32508/stdj.v19i4.652
Published: 2016-12-31

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Copyright © 2016 by the author(s).

Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

FliC protein from Salmonella enteritidis is currently interested due to its immunologic adjuvant property for the novel generation of recombinant vaccines. To produce a source for further researches on the immune effects of FliC, we generated an Escherichia coli based on recombinant vector called pET-fliC which is ligated from fliC gene with NdeI and XhoI double digested pET vectors. The results of expression of recombinant FliC, which was induced by IPTG, were confirmed by SDS-PAGE and Western blot probed with anti-6xHis tag. With the purity above 95 %, this recombinant FliC can be used as a material source for next studies on evaluating the adjuvant property of FliC.

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