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Abstract
In cantilever-based biosensor, Au surface plays two essential roles: as a surface to reflect laser beam and as a surface to be modified and thus functionalize the sensor. In this paper, we researched on modifying the Au surface by cysteamine and glutaraldehyde to make it reactive toward amine substances. Cysteamine concentration, cysteamine treatment time and glutaraldehyde treatment time were investigated to find optimal values. The data of chromogenic reaction catalyzed by horseradish peroxidase (HRP) and the data of water contact angle measurement were combined to find the optimal values. The results showed that the modification with 5 mM cysteamine in ethanol for 16 h and glutaraldehyde for 1 h would create the Au surface which can react optimally with amine substances.
Issue: Vol 19 No 4 (2016)
Page No.: 178-187
Published: Dec 31, 2016
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v19i4.691
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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