Effect of plant hormones on the in vitro culture of breadfruit shoots (Artocarpus altilis (Park.) Fosberg)

Abstract

Under the influence of plant hormones, after 8 weeks of in vitro culture, the growth of breadfruit shoot (Artocarpus altilis (Park.) Fosberg) was very different. With shoots that were cultured on 1 mg/L BA medium after 10 days of being transferred to ½ MS supplemented with 10 mg/L BA medium, the percentage of shoot development was the highest (86.8 %), and the secretion of phenolic compounds or forming callus were not observed. On the ½ MS supplemented with 12 mg/L BA medium, shoots growed strongly and healthily but the secretion of phenolic compounds and the forming of callus affected the ability of the shoot development. On the ½ MS supplemented with 0.45 mg/L BA, 0.6 mg/L Kinetin (Kin) medium and on the ½ MS supplemented with 0.45 mg/L BA, 0.6 mg/L Kin, 0.35 mg/L GA3 medium, the percentage of shoot developed were lower than those on the ½ MS supplemented with 10 mg/L medium. The addition of 0.35 mg/L GA3 in the culture medium help to appear the lateral more than the remaining experiments. Roles of respiration rate and endogenous hormones were discussed to understand the physiological changes in the in vitro culture shoots breadfruit.