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In this work, ultrasonic treatment was used for improving the catalytic activity of glucoamylase preparation Dextrozyme GA. The ultrasonic temperature, power and time were optimized by a Central Composite Circumscribed design for maximizing of the catalytic activity of the preparation. The optimal ultrasonic temperature, power and time were 30oC, 20 W/mL and 33 sec, respectively. Under these conditions, the maximum glucoamylase activity was 83.142 ± 0.213 KU/mL and this value increased 11 % in comparison with that in the control without ultrasonic treatment. Our results also showed that Vmax and KM of the sonicated Dextrozyme GA preparation were higher than those of the control. The ultrasonic treatment would be a potential method for improving the catalytic activity of the glucoamylase preparation in starch hydrolysis.

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Issue: Vol 18 No 3 (2015)
Page No.: 52-59
Published: Aug 30, 2015
Section: Engineering and Technology - Research article

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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Tran, T., & Le, M. (2015). Optimization of the ultrasonic treatment for improving catalytic activity of glucoamylase preparation. Science and Technology Development Journal, 18(3), 52-59.

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