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VNUHCM Journal of

Science and Technology Development

An official journal of Viet Nam National University Ho Chi Minh City, Viet Nam since 1997

ISSN: 1859-0128
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Engineering and Technology - Research article Open Access Logo

Optimization of Chitosan hydrolysis by Cellulase Enzyme to produce Chitooligosaccharide

Hoai Van Bui 1, *
Quang An Dao 2
Nghiep Dai Ngo 3

  1. VNUHCM-University of Science and Center of Experiment and Practice,Ho Chi Minh City University of Food Industry
  2. Center of Experiment and Practice,Ho Chi Minh City University of Food Industry
  3. VNUHCM-University of Science
Correspondence to: Hoai Van Bui, VNUHCM-University of Science and Center of Experiment and Practice,Ho Chi Minh City University of Food Industry. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 20 No. K3 (2017) | Page No.: 74-82 | DOI: 10.32508/stdj.v20iK3.1095
Published: 2017-06-30

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Copyright © 2017 by the author(s).

Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

In order to increase the water solubility of chitosan and potential application to products that is good for human health. Chitosan was caried out the optimization of hydrolysis by cellulase to produce chitooligosaccharide. Chitosan with degree of deacetyl more than 80% and cellulase used in this study. Surface Response Method (RSM) - Central Composite Design (CCD) option is used to optimize the hydrolysis. Results of the study showed optimal values for hydrolysis such as 49 oC temperature, 5.9 pH, 0.76% substrate concentration, and 8.97 U/g enzyme concentration, 180 minutes hydrolysis time. More than 90% of the oligosaccharides produced were in the range less than 10 kDa. The research results are the premise for production of COS powder to water-soluble.

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