Inhitive ability collagenase of N-(cinnamyl) chitooligosaccharide derivative

Abstract

Chitooligosaccharides (COS) with molecular weight 4633 Da and 84.67% of deacetylation were synthesized by hydrolysis of chitosan by cellulase at room temperature (33 ± 1 ° C). This COS, then, were chemically modified by grafting cinnamaldehyde at amino groups on the COS. Derivatives of N- (cinnamyl) chitooligosaccharides (CCOS) synthesized with 50.64% of yield and 72.22% of extent of substitution had inhibitory activity enzyme collagenase (a group of matrix metalloproteinases, enzyme family related to metastatic ability of cancer). Compared with the positive control, 58.23% of the effective to inhibit collagenase of CCOS at 1000 μg/ml concentrate. In addition, the CCOS cytotoxicity of CCOS was also assessed by MTT method, the results showed that non-toxic derivatives of animal cells and thus can be tested and applied in living systems.