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Somatic embryogenesis from Hoan ngoc(Pseuderanthemum palatiferum (Ness) Radlk) leaf callus

Nhung Thi Tuyet Tran 1, *
Hoang Ngo Phan 1
Sanh Du Nguyen 1
  1. University of Science, VNU-HCM
Correspondence to: Nhung Thi Tuyet Tran, University of Science, VNU-HCM. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 17 No. 2 (2014) | Page No.: 100-107 | DOI: 10.32508/stdj.v17i2.1319
Published: 2014-06-30

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This article is published with open access by Viet Nam National University, Ho Chi Minh City, Viet Nam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Abstract

Pseuderanthemum palatiferum contains many compounds which have pharmaceutical and medicinal values. In this study, the in vitro somatic embryogenesis of callus formed from Pseuderanthemum palatiferum’s leaves was performed and analyzed. The calli were induced in three weeks in MS medium with 1 mg.l-1 or 2 mg.l-1 of 2,4-D. The somatic embryos were developed as follows: the globular embryos formed after 9 days, the heart embryos formed after 13 days, the torpedo embryos formed after 15 days and the cotyledonary embryos formed after 17 days. The mean number of obtained embryos was 28.5 embryos/cm2 of leaf tissue. The intensity of respiration and biological activity of endogenous plant growth regulators of leaf tissue during culture were recorded.

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