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VNUHCM Journal of

Science and Technology Development

An official journal of Viet Nam National University Ho Chi Minh City, Viet Nam since 1997

ISSN: 1859-0128
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Study on synthesis of colloidal gold nanoparticles and preparation of antiE. coli O157 monoclonal antibody conjugated nanoparticles

Thanh Vo Ke Ngo 1, *
Giang Dang Nguyen 1
Vinh Quang Lam 2
Dat Thanh Huynh 3

  1. Integrated Circuit Design Research & Education Center, VNU-HCM
  2. University of Science, VNU-HCM
  3. Viet Nam National University - Ho Chi Minh City
Correspondence to: Thanh Vo Ke Ngo, Integrated Circuit Design Research & Education Center, VNU-HCM. Email: pvphuc@vnuhcm.edu.vn.
Volume & Issue: Vol. 16 No. 3 (2013) | Page No.: 75-82 | DOI: 10.32508/stdj.v16i3.1640
Published: 2013-09-30

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Copyright © 2013 by the author(s).

Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

The present paper describes the preparation of colloidal gold nanoparticles by a chemical reduction method, using some chemical compounds such as sodium citrate (Na3Ct), chloroauric acid (HAuCl4.3H2O) and anti-E. coli O157 monoclonal antibodyconjugated gold nanoparticles. The nanoparticles were characterized by several techniques including Ultraviolet and Visible Absorption Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM). The UV-Vis confirmed that the concentration of Na3Ct has a major effect on the size of the synthesized gold nanoparticles, and TEM images showed that the size of gold particles is in the range of 10-20 nm. In addition, the binding of the antibody-gold conjugates to E. coli O157 was also investigated using the same characterization means

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