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Abstract

Extracellular matrices (ECM) have been reported to enhance cell attachment and proliferation as well as to create stem cell niches invitro. We harvested ECM from human fibroblasts for a number of researches, including tissue engineering. Fibroblasts were isolated from human foreskins, cultured in DMEMF12 containing 10% FBS and identified by Trichrome staining and immunohistochemistry for Vimentin. Then, fibroblasts were stimulated to synthetize ECM in medium supplemented 0.05% ascorbic acid. Cell constituents were removed by using Triton X-100, NH4OH and DNAse. ECM proteins were evaluated by PAS staining. Results showed that collagen is present in ECM.

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Issue: Vol 12 No 9 (2009)
Page No.: 5-11
Published: May 15, 2009
Section: Article
DOI: https://doi.org/10.32508/stdj.v12i9.2280

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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Nguyen, G., To, Q., Phan, N., & Tran, H. (2009). COLLECT EXTRACELLULAR MATRIX FROM IN VITRO FIBROBLAST. Science and Technology Development Journal, 12(9), 5-11. https://doi.org/https://doi.org/10.32508/stdj.v12i9.2280


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