VEGETATIVE MULTIPLICATION OF SAINTPAULIA BY IN-VITRO METHOD

Abstract

The aim of the paper is to present some results in the study on the conditions of samples sterilization, appropriate nutrient solutions for the cell transformation and the organogenesis in rapid vegetative multiplication of Saintpaulia by using in-vitro culture. The obtained results point out that: Sterilization with 10 % CaOCl2 solution in 10 minutes or 0,1 % HgCl2 solution in 5 minutes gives the least contaminated samples. The samples from leaf form a lot of vegetative buds at the leaf border and those from petiole create vegetative buds concentrated at vein region. Murashige and Skoog (MS) medium supplemented with IAA 0,5 mg/l and BA 2,5 mg/l is the most appropriate for bud formation whereas MS medium supplemented with IAA 0,5 mg/l is the most appropriate for root formation. These results are profitable for the rapid in-vitro culture of Saintpaulia.